› 4wn4

X-ray diffraction
3.85Å resolution

Crystal structure of designed cPPR-polyA protein

Released:
DOI: 10.2210/pdb4wn4/pdb
PDB entry 4wn4 coloured by chain, front view.
PDB entry 4wn4 coloured by chain, side view.
PDB entry 4wn4 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
An artificial PPR scaffold for programmable RNA recognition.
Coquille S, Filipovska A, Chia T, Rajappa L, Lingford JP, Razif MF, Thore S, Rackham O
Nat Commun 5 5729 (2014)
PMID: 25517350

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pentatricopeptide repeat protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 33.23 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: F23
Unit cell:
a: 204.72Å b: 204.72Å c: 204.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.179 0.23
Expression system: Escherichia coli

Quick links

Citations

25 review citations

The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.
Jourdain et al. (2017)
24 more