› 4wrd

X-ray diffraction
1.65Å resolution

Crystal structure of Staphylcoccal nulease variant Delta+PHS V66E L125E at cryogenic temperature

Released:
DOI: 10.2210/pdb4wrd/pdb
PDB entry 4wrd coloured by chain, front view.
PDB entry 4wrd coloured by chain, side view.
PDB entry 4wrd coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Entry authors: Bell-Upp PC, Schlessman JL, Garcia-Moreno E B

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermonuclease Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.19 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5HHM4 (Residues: 80-228; Coverage: 70%)
Gene names: SACOL0860, nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21
Unit cell:
a: 31.14Å b: 60.315Å c: 38.364Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli BL21(DE3)

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