› 4wsi

X-ray diffraction
2.95Å resolution

Crystal Structure of PALS1/Crb complex

Released:
DOI: 10.2210/pdb4wsi/pdb
PDB entry 4wsi coloured by chain, front view.
PDB entry 4wsi coloured by chain, side view.
PDB entry 4wsi coloured by chain, top view.
Source organisms:
Primary publication:
Structure of Crumbs tail in complex with the PALS1 PDZ-SH3-GK tandem reveals a highly specific assembly mechanism for the apical Crumbs complex.
Li Y, Wei Z, Yan Y, Wan Q, Du Q, Zhang M
Proc Natl Acad Sci U S A 111 17444-9 (2014)
PMID: 25385611

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145148 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein PALS1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 44.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N3R9 (Residues: 236-675; Coverage: 58%)
Gene names: MPP5, PALS1
Sequence domains:
Structure domains:
Protein crumbs Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 43 amino acids
Theoretical weight: 4.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P10040 (Residues: 2110-2146; Coverage: 2%)
Gene names: CG6383, crb

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 111.928Å b: 111.928Å c: 223.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.235
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Mechanistic basis of MAGUK-organized complexes in synaptic development and signalling.
Zhu et al. (2016)
9 more

9 mentions without citation

Structural biology of SARS-CoV-2: open the door for novel therapies.
Yan et al. (2022)
8 more