› 4wv8

X-ray diffraction
1.83Å resolution

Crystal structure of a recombinant Vatairea macrocarpa seed lectin complexed with lactose

Released:
DOI: 10.2210/pdb4wv8/pdb
PDB entry 4wv8 coloured by chain, front view.
PDB entry 4wv8 coloured by chain, side view.
PDB entry 4wv8 coloured by chain, top view.
Source organism: Vatairea macrocarpa
Primary publication:
Structural characterization of a Vatairea macrocarpa lectin in complex with a tumor-associated antigen: A new tool for cancer research.
Sousa BL, Silva-Filho JC, Kumar P, Graewert MA, Pereira RI, Cunha RMS, Nascimento KS, Bezerra GA, Delatorre P, Djinovic-Carugo K, Nagano CS, Gruber K, Cavada BS
Int J Biochem Cell Biol 72 27-39 (2016)
PMID: 26751394

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Seed lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 241 amino acids
Theoretical weight: 26.35 KDa
Source organism: Vatairea macrocarpa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P81371 (Residues: 1-240; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, GAL
Carbohydrate polymer
2 bound ligands:
Ligand CA 4 x CA
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 152.644Å b: 97.427Å c: 78.667Å
α: 90° β: 94.68° γ: 90°
R-values:
R R work R free
0.159 0.157 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Molecular Basis for Recognition of the Cancer Glycobiomarker, LacdiNAc (GalNAc[β1→4]GlcNAc), by Wisteria floribunda Agglutinin.
Haji-Ghassemi et al. (2016)
5 more