› 4wxm

X-ray diffraction
2.3Å resolution

FleQ REC domain from Pseudomonas aeruginosa PAO1

Released:
DOI: 10.2210/pdb4wxm/pdb
PDB entry 4wxm coloured by chain, front view.
PDB entry 4wxm coloured by chain, side view.
PDB entry 4wxm coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The REC domain mediated dimerization is critical for FleQ from Pseudomonas aeruginosa to function as a c-di-GMP receptor and flagella gene regulator.
Su T, Liu S, Wang K, Chi K, Zhu D, Wei T, Huang Y, Guo L, Hu W, Xu S, Lin Z, Gu L
J Struct Biol 192 1-13 (2015)
PMID: 26362077

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator FleQ Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 143 amino acids
Theoretical weight: 16.27 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XCV0 (Residues: 1-139; Coverage: 28%)
Gene names: PA1097, fleQ
Sequence domains: Flagellar regulatory protein FleQ
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 144.397Å b: 58.451Å c: 92.213Å
α: 90° β: 118.35° γ: 90°
R-values:
R R work R free
0.214 0.21 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Biofilms and Cyclic di-GMP (c-di-GMP) Signaling: Lessons from Pseudomonas aeruginosa and Other Bacteria.
Valentini et al. (2016)
4 more