› 4x59

X-ray diffraction
1.8Å resolution

Anthranilate phosphoribosyltransferase variant P180A from Mycobacterium tuberculosis in complex with PRPP and Mg

Released:
DOI: 10.2210/pdb4x59/pdb
PDB entry 4x59 coloured by chain, front view.
PDB entry 4x59 coloured by chain, side view.
PDB entry 4x59 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structures of Mycobacterium tuberculosis Anthranilate Phosphoribosyltransferase Variants Reveal the Conformational Changes That Facilitate Delivery of the Substrate to the Active Site.
Cookson TV, Evans GL, Castell A, Baker EN, Lott JS, Parker EJ
Biochemistry 54 6082-92 (2015)
PMID: 26356348

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 38.82 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX5 (Residues: 1-370; Coverage: 100%)
Gene names: MTCY190.03c, Rv2192c, trpD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PRP 2 x PRP
Ligand GOL 6 x GOL
Ligand MG 2 x MG
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 95.234Å b: 78.339Å c: 102.155Å
α: 90° β: 111.74° γ: 90°
R-values:
R R work R free
0.191 0.19 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
4 more

2 mentions without citation

Motions around conserved helical weak spots facilitate GPCR activation.
Bibbe et al. (2021)
1 more