› 4x7e

X-ray diffraction
2.11Å resolution

Crystal structure of norovirus GII.10 P domain in complex with Nano-85

Released:
DOI: 10.2210/pdb4x7e/pdb
PDB entry 4x7e coloured by chain, front view.
PDB entry 4x7e coloured by chain, side view.
PDB entry 4x7e coloured by chain, top view.
Source organisms:
Primary publication:
Nanobody binding to a conserved epitope promotes norovirus particle disassembly.
Koromyslova AD, Hansman GS
J Virol 89 2718-30 (2015)
PMID: 25520510

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212305 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.51 KDa
Source organism: Norovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5F4T5 (Residues: 224-538; Coverage: 58%)
Sequence domains: Calicivirus coat protein C-terminal
Structure domains:
Nano-85 Nanobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 126 amino acids
Theoretical weight: 13.5 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 51.94Å b: 133.1Å c: 67.57Å
α: 90° β: 112.54° γ: 90°
R-values:
R R work R free
0.19 0.189 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

10 review citations

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells.
Dmitriev et al. (2016)
9 more

2 mentions without citation

Nanobody binding to a conserved epitope promotes norovirus particle disassembly.
Koromyslova et al. (2015)
1 more