› 4xa3

X-ray diffraction
2.55Å resolution

Crystal structure of the coiled-coil surrounding Skip 2 of MYH7

Released:
DOI: 10.2210/pdb4xa3/pdb
PDB entry 4xa3 coloured by chain, front view.
PDB entry 4xa3 coloured by chain, side view.
PDB entry 4xa3 coloured by chain, top view.
Source organisms:
Primary publication:
Skip residues modulate the structural properties of the myosin rod and guide thick filament assembly.
Taylor KC, Buvoli M, Korkmaz EN, Buvoli A, Zheng Y, Heinze NT, Cui Q, Leinwand LA, Rayment I
Proc Natl Acad Sci U S A 112 E3806-15 (2015)
PMID: 26150528

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid assembly scaffolding protein; Microtubule-associated protein RP/EB family member 1; Myosin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 17.77 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13848 (Residues: 1-49; Coverage: 50%)
  • Canonical: P12883 (Residues: 1361-1425; Coverage: 3%)
  • Canonical: Q15691 (Residues: 215-251; Coverage: 14%)
Gene names: 7, MAPRE1, MYH7, MYHCB
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 37.462Å b: 146.115Å c: 153.443Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.267 0.311
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Disease mutations in striated muscle myosins.
Parker et al. (2020)
1 more

7 mentions without citation

The accommodation index measures the perturbation associated with insertions and deletions in coiled-coils: Application to understand signaling in histidine kinases.
Schmidt et al. (2017)
6 more