› 4xah

X-ray diffraction
2.5Å resolution

X-ray crystal structure of S. cerevisiae Cgi121

Released:
DOI: 10.2210/pdb4xah/pdb
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PDB entry 4xah coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.
Zhang W, Collinet B, Graille M, Daugeron MC, Lazar N, Libri D, Durand D, van Tilbeurgh H
OpenAccess logo Nucleic Acids Res 43 3358-72 (2015)
PMID: 25735745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EKC/KEOPS complex subunit CGI121 Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 21.59 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q03705 (Residues: 1-181; Coverage: 100%)
Gene names: CGI121, YML036W
Sequence domains: Kinase binding protein CGI-121
Structure domains: CGI121/TPRKB

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 75.65Å b: 76.9Å c: 84.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.236
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

2 review citations

Conservation and Diversification of tRNA t6A-Modifying Enzymes across the Three Domains of Life.
Su et al. (2022)
1 more

1 mention without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)

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