› 4xbe

X-ray diffraction
1.76Å resolution

Crystal structure of human 4E10 Fab in complex with its peptide epitope on HIV-1 gp41: crystals cryoprotected with sphingomyelin (02:0 SM (d18:1/2:0)).

Released:
DOI: 10.2210/pdb4xbe/pdb
Model geometry
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PDB entry 4xbe coloured by chain, front view.
PDB entry 4xbe coloured by chain, side view.
PDB entry 4xbe coloured by chain, top view.
Source organisms:
Primary publication:
Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Irimia A, Sarkar A, Stanfield RL, Wilson IA
Immunity 44 21-31 (2016)
PMID: 26777395

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229850 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
4E10 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
4E10 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: P

Ligands and Environments

1 bound ligand:
Ligand UNL 2 x UNL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 157.657Å b: 44.515Å c: 85.344Å
α: 90° β: 113.48° γ: 90°
R-values:
R R work R free
0.153 0.15 0.196
Expression systems:
  • Homo sapiens
  • Not provided

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Citations

14 review citations

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
13 more

6 mentions without citation

The biology of ergothioneine, an antioxidant nutraceutical.
Borodina et al. (2020)
5 more

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