› 4xfa

X-ray diffraction
1.65Å resolution

cysteine dioxygenase variant - Y157F at pH 8.0 in complex with homocysteine

Released:
DOI: 10.2210/pdb4xfa/pdb
PDB entry 4xfa coloured by chain, front view.
PDB entry 4xfa coloured by chain, side view.
PDB entry 4xfa coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Driggers CM, Karplus PA

Function and Biology Details

Reaction catalysed: 
L-cysteine + O(2) = 3-sulfinoalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine dioxygenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 23.04 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21816 (Residues: 1-200; Coverage: 100%)
Gene name: Cdo1
Sequence domains: Cysteine dioxygenase type I
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand HCS 1 x HCS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 57.6Å b: 57.6Å c: 122.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.169 0.21
Expression system: Escherichia coli

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