› 4xiy

X-ray diffraction
2.5Å resolution

Crystal structure of ketol-acid reductoisomerase from Azotobacter

Released:
DOI: 10.2210/pdb4xiy/pdb
PDB entry 4xiy coloured by chain, front view.
PDB entry 4xiy coloured by chain, side view.
PDB entry 4xiy coloured by chain, top view.
Source organism: Azotobacter vinelandii DJ
Primary publication:
Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Cahn JK, Brinkmann-Chen S, Spatzal T, Wiig JA, Buller AR, Einsle O, Hu Y, Ribbe MW, Arnold FH
Biochem J 468 475-84 (2015)
PMID: 25849365

Function and Biology Details

Reaction catalysed: 
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ketol-acid reductoisomerase (NADP(+)) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 338 amino acids
Theoretical weight: 36.68 KDa
Source organism: Azotobacter vinelandii DJ
UniProt:
  • Canonical: C1DFH7 (Residues: 1-338; Coverage: 100%)
Gene names: Avin_42500, ilvC
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand FE 4 x FE
Ligand PGE 2 x PGE
Ligand PEG 1 x PEG
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P213
Unit cell:
a: 185.05Å b: 185.05Å c: 185.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.244

Quick links

Citations

1 review citation

Bacterial Branched-Chain Amino Acid Biosynthesis: Structures, Mechanisms, and Drugability.
Amorim Franco et al. (2017)
8 other articles

2 mentions without citation

Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases.
Cahn et al. (2015)
1 more