› 4xk1

X-ray diffraction
2.15Å resolution

Crystal Structure of a Phosphoserine/phosphohydroxythreonine Aminotransferase (PSAT) from Pseudomonas aeruginosa with cofactor Pyridoxal Phosphate and bound Glutamate

Released:
DOI: 10.2210/pdb4xk1/pdb
PDB entry 4xk1 coloured by chain, front view.
PDB entry 4xk1 coloured by chain, side view.
PDB entry 4xk1 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191179 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 41.25 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HZ66 (Residues: 1-361; Coverage: 100%)
Gene names: PA3167, serC
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FMT 3 x FMT
Ligand GLU 1 x GLU
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 72.22Å b: 72.22Å c: 268.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.219
Expression system: Escherichia coli BL21(DE3)

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