› 4xoo

X-ray diffraction
2.1Å resolution

FMN complex of coenzyme F420:L-glutamate ligase (FbiB) from Mycobacterium tuberculosis (C-terminal domain)

Released:
DOI: 10.2210/pdb4xoo/pdb
PDB entry 4xoo coloured by chain, front view.
PDB entry 4xoo coloured by chain, side view.
PDB entry 4xoo coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Elongation of the Poly-γ-glutamate Tail of F420 Requires Both Domains of the F420:γ-Glutamyl Ligase (FbiB) of Mycobacterium tuberculosis.
Bashiri G, Rehan AM, Sreebhavan S, Baker HM, Baker EN, Squire CJ
J Biol Chem 291 6882-94 (2016)
PMID: 26861878

Function and Biology Details

Reactions catalysed: 
Oxidized coenzyme F420-0 + FMN = dehydro coenzyme F420-0 + FMNH(2)
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional F420 biosynthesis protein FbiB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 22.35 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WP79 (Residues: 245-448; Coverage: 46%)
Gene names: Rv3262, fbiB
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

1 bound ligand:
Ligand FMN 4 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P41212
Unit cell:
a: 137.135Å b: 137.135Å c: 101.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.232 0.269
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

The methanogenic redox cofactor F420 is widely synthesized by aerobic soil bacteria.
Ney et al. (2017)
6 more

4 mentions without citation

Evolutionary and molecular foundations of multiple contemporary functions of the nitroreductase superfamily.
Akiva et al. (2017)
3 more