› 4xva

X-ray diffraction
2.66Å resolution

Crystal structure of wild type cytochrome c peroxidase

Released:
DOI: 10.2210/pdb4xva/pdb
PDB entry 4xva coloured by chain, front view.
PDB entry 4xva coloured by chain, side view.
PDB entry 4xva coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
One Crystal, Two Temperatures: Cryocooling Penalties Alter Ligand Binding to Transient Protein Sites.
Fischer M, Shoichet BK, Fraser JS
OpenAccess logo Chembiochem 16 1560-4 (2015)
PMID: 26032594

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c peroxidase, mitochondrial Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 293 amino acids
Theoretical weight: 33.47 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00431 (Residues: 69-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:
Ligand BZI 3 x BZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 84.11Å b: 104.76Å c: 185.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.233 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Crystallography and Its Impact on Carbonic Anhydrase Research.
Lomelino et al. (2018)
2 more