› 4y12

X-ray diffraction
1.9Å resolution

Crystal structure of the S/T protein kinase PknG from Mycobacterium tuberculosis in complex with AGS

Released:
DOI: 10.2210/pdb4y12/pdb
PDB entry 4y12 coloured by chain, front view.
PDB entry 4y12 coloured by chain, side view.
PDB entry 4y12 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Molecular Basis of the Activity and the Regulation of the Eukaryotic-like S/T Protein Kinase PknG from Mycobacterium tuberculosis.
Lisa MN, Gil M, André-Leroux G, Barilone N, Durán R, Biondi RM, Alzari PM
Structure 23 1039-48 (2015)
PMID: 25960409

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknG Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 40.96 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI73 (Residues: 74-405; Coverage: 44%)
Gene names: MTCY22G10.06c, Rv0410c, pknG
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand AGS 1 x AGS
Ligand ZN 1 x ZN
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 75.452Å b: 37.183Å c: 108.032Å
α: 90° β: 97.93° γ: 90°
R-values:
R R work R free
0.196 0.196 0.212
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

The exploitation of host autophagy and ubiquitin machinery by Mycobacterium tuberculosis in shaping immune responses and host defense during infection.
Shariq et al. (2023)
3 more

5 mentions without citation

NU-6027 Inhibits Growth of Mycobacterium tuberculosis by Targeting Protein Kinase D and Protein Kinase G.
Kidwai et al. (2019)
4 more