› 4y1q

X-ray diffraction
3.1Å resolution

Crystal Structure of HasA mutant Y75A monomer from Yersinia pseudotuberculosis

Released:
DOI: 10.2210/pdb4y1q/pdb
PDB entry 4y1q coloured by chain, front view.
PDB entry 4y1q coloured by chain, side view.
PDB entry 4y1q coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis IP 32953
Primary publication:
The crystal structure of heme acquisition system A from Yersinia pseudotuberculosis (HasAypt): Roles of the axial ligand Tyr75 and two distal arginines in heme binding.
Kanadani M, Sato T, Hino T, Nagano S, Ozaki S
J Inorg Biochem 151 26-33 (2015)
PMID: 26210321

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular heme acquisition hemophore HasA Chains: A, B, D, G, I, K, M, O
Molecule details ›
Chains: A, B, D, G, I, K, M, O
Length: 217 amino acids
Theoretical weight: 23.56 KDa
Source organism: Yersinia pseudotuberculosis IP 32953
Expression system: Escherichia coli
UniProt:
  • Canonical: Q66G68 (Residues: 1-205; Coverage: 100%)
Gene names: HasA, YPTB0114
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

1 bound ligand:
Ligand HEM 8 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P1
Unit cell:
a: 69.66Å b: 70.32Å c: 77.03Å
α: 89.35° β: 89.75° γ: 90.46°
R-values:
R R work R free
0.222 0.22 0.276
Expression system: Escherichia coli

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