› 4y21

X-ray diffraction
2.9Å resolution

Crystal Structure of Munc13-1 MUN domain

Released:
DOI: 10.2210/pdb4y21/pdb
PDB entry 4y21 coloured by chain, front view.
PDB entry 4y21 coloured by chain, side view.
PDB entry 4y21 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming.
Yang X, Wang S, Sheng Y, Zhang M, Zou W, Wu L, Kang L, Rizo J, Zhang R, Xu T, Ma C
Nat Struct Mol Biol 22 547-54 (2015)
PMID: 26030875

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein unc-13 homolog A Chain: A
Molecule details ›
Chain: A
Length: 539 amino acids
Theoretical weight: 61.99 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62768 (Residues: 942-1407, 1453-1523; Coverage: 31%)
  • Best match: Q62768-2 (Residues: 942-1407, 1410-1500)
Gene names: Unc13a, Unc13h1
Sequence domains: MUN domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 114.062Å b: 270.921Å c: 47.735Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Chaperoning SNARE assembly and disassembly.
Baker et al. (2016)
29 more

6 mentions without citation

On the difficulties of characterizing weak protein interactions that are critical for neurotransmitter release.
Rizo et al. (2022)
5 more