› 4y5d

X-ray diffraction
1.2Å resolution

CRYSTAL STRUCTURE OF ALiS2-STREPTAVIDIN COMPLEX

Released:
DOI: 10.2210/pdb4y5d/pdb
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PDB entry 4y5d coloured by chain, front view.
PDB entry 4y5d coloured by chain, side view.
PDB entry 4y5d coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport.
Terai T, Kohno M, Boncompain G, Sugiyama S, Saito N, Fujikake R, Ueno T, Komatsu T, Hanaoka K, Okabe T, Urano Y, Perez F, Nagano T
J Am Chem Soc 137 10464-7 (2015)
PMID: 26261872

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 12.85 KDa
Source organism: Streptomyces avidinii
Expression system: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 39-160; Coverage: 77%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

4 bound ligands:
Ligand MT6 3 x MT6
Ligand PE3 1 x PE3
Ligand DMS 1 x DMS
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 57.927Å b: 83.774Å c: 45.947Å
α: 90° β: 98.75° γ: 90°
R-values:
R R work R free
0.14 0.138 0.174
Expression system: Streptomyces avidinii

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Citations

2 review citations

Synthetic mimics of biotin/(strept)avidin.
Liu et al. (2017)
1 more

2 mentions without citation

First-Principles Collision Cross Section Measurements of Large Proteins and Protein Complexes.
McCabe et al. (2020)
1 more

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