4yiz

X-ray diffraction
2.2Å resolution

Crystal structure of engineered TgAMA1 lacking the DII loop in complex with an Eimeria tenella RON2D3 peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-197470 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apical membrane antigen AMA1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 419 amino acids
Theoretical weight: 46.06 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Trichoplusia ni
UniProt:
  • Canonical: S8GKS3 (Residues: 64-339, 360-484; Coverage: 71%)
Gene name: TGME49_255260
Sequence domains: Apical membrane antigen 1
Structure domains:
Rhoptry neck protein 2, putative Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 40 amino acids
Theoretical weight: 3.87 KDa
Source organism: Eimeria tenella
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: U6KQJ2 (Residues: 1261-1297; Coverage: 3%)
Gene name: ETH_00012760

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3221
Unit cell:
a: 265.928Å b: 265.928Å c: 94.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.187 0.207
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)