› 4yj1

X-ray diffraction
2.05Å resolution

Crystal structure of T. brucei MRB1590-ADP bound to poly-U RNA

Released:
DOI: 10.2210/pdb4yj1/pdb
PDB entry 4yj1 coloured by chain, front view.
PDB entry 4yj1 coloured by chain, side view.
PDB entry 4yj1 coloured by chain, top view.
Source organism: Trypanosoma brucei brucei TREU927
Primary publication:
Structures of the T. brucei kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold.
Shaw PL, McAdams NM, Hast MA, Ammerman ML, Read LK, Schumacher MA
OpenAccess logo Nucleic Acids Res 43 7096-109 (2015)
PMID: 26117548

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial RNA binding complex 1 subunit Chain: A
Molecule details ›
Chain: A
Length: 615 amino acids
Theoretical weight: 67.05 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57ZF2 (Residues: 50-665; Coverage: 92%)
Gene names: Tb03.30P12.70, Tb927.3.1590
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I422
Unit cell:
a: 215.433Å b: 215.433Å c: 100.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.203
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.
Aphasizheva et al. (2020)
2 more

4 mentions without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)
3 more