› 4yje

X-ray diffraction
1.9Å resolution

Crystal structure of APC-ARM in complexed with Amer1-A1

Released:
DOI: 10.2210/pdb4yje/pdb
PDB entry 4yje coloured by chain, front view.
PDB entry 4yje coloured by chain, side view.
PDB entry 4yje coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the APC-ARM domain in complexes with discrete Amer1/WTX fragments reveal that it uses a consensus mode to recognize its binding partners.
Zhang Z, Akyildiz S, Xiao Y, Gai Z, An Y, Behrens J, Wu G
OpenAccess logo Cell Discov 1 15016 (2015)
PMID: 27462415

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-540859 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Adenomatous polyposis coli protein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25054 (Residues: 407-751; Coverage: 12%)
Gene names: APC, DP2.5
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
APC membrane recruitment protein 1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.12 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q5JTC6 (Residues: 325-335; Coverage: 1%)
Gene names: AMER1, FAM123B, WTX

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 53.205Å b: 68.204Å c: 93.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.221
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Advances and Insights of APC-Asef Inhibitors for Metastatic Colorectal Cancer Therapy.
Yang et al. (2021)
4 other articles

2 mentions without citation

The structural biology of canonical Wnt signalling.
Agostino et al. (2020)
1 more