› 4yjf

X-ray diffraction
2.2Å resolution

Crystal structure of DAAO(Y228L/R283G) variant (S-methylbenzylamine binding form)

Released:
DOI: 10.2210/pdb4yjf/pdb
PDB entry 4yjf coloured by chain, front view.
PDB entry 4yjf coloured by chain, side view.
PDB entry 4yjf coloured by chain, top view.
Source organism: Sus scrofa
Entry authors: Nakano S, Yasukawa K, Kawahara N, Ishitsubo E, Tokiwa H, Asano Y

Function and Biology Details

Reaction catalysed: 
A D-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132444 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
D-amino-acid oxidase Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.56 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00371 (Residues: 1-341; Coverage: 98%)
Gene name: DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:
D-amino-acid oxidase Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 38.35 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00371 (Residues: 1-339; Coverage: 98%)
Gene name: DAO
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand SO4 4 x SO4
Ligand 98B 2 x 98B
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 68.844Å b: 91.973Å c: 110.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.226
Expression system: Escherichia coli

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