4yl6

X-ray diffraction
2.1Å resolution

Crystal structure of truncated cerebral cavernous malformation 2 C-terminal adaptor domain in complex with an internal helix of mitogen-activated protein kinase kinase kinase 3

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-573070 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cerebral cavernous malformations 2 protein Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 11.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BSQ5 (Residues: 290-376; Coverage: 20%)
Gene names: C7orf22, CCM2, PP10187
Sequence domains: Cerebral cavernous malformation protein, harmonin-homology
Structure domains: Paired amphipathic helix 2 (pah2 repeat)
Mitogen-activated protein kinase kinase kinase 3 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.59 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q99759 (Residues: 1-22; Coverage: 4%)
Gene names: MAP3K3, MAPKKK3, MEKK3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P43212
Unit cell:
a: 61.21Å b: 61.21Å c: 68.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided