› 4ync

X-ray diffraction
1.5Å resolution

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 44.57 KDa
Source organism: Saccharomyces pastorianus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02899 (Residues: 2-398; Coverage: 99%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand 4EG 1 x 4EG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P43212
Unit cell:
a: 141.503Å b: 141.503Å c: 42.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.172
Expression system: Escherichia coli BL21(DE3)

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