› 4yqz

X-ray diffraction
1.81Å resolution

Crystal Structure of a putative oxidoreductase from Thermus Thermophilus HB27 (TT_P0034, TARGET EFI-513932) in its APO form

Released:
DOI: 10.2210/pdb4yqz/pdb
PDB entry 4yqz coloured by chain, front view.
PDB entry 4yqz coloured by chain, side view.
PDB entry 4yqz coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Entry authors: Yadava U, Vetting MW, Al Obaidi NF, Toro R, Morisco LL, Benach J, Koss J, Wasserman SR, Attonito JD, Scott Glenn A, Chamala S, Chowdhury S, Lafleur J, Love J, Seidel RD, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative oxidoreductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 27.51 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q746L9 (Residues: 2-234; Coverage: 100%)
Gene name: TT_P0034
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 76.436Å b: 65.752Å c: 85.759Å
α: 90° β: 95.43° γ: 90°
R-values:
R R work R free
0.167 0.164 0.21
Expression system: Escherichia coli BL21(DE3)

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