› 4yw9

X-ray diffraction
1.4Å resolution

Structure of rat cytosolic pepck in complex with 3-mercaptopicolinic acid and GTP

Released:
DOI: 10.2210/pdb4yw9/pdb
PDB entry 4yw9 coloured by chain, front view.
PDB entry 4yw9 coloured by chain, side view.
PDB entry 4yw9 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Inhibition and Allosteric Regulation of Monomeric Phosphoenolpyruvate Carboxykinase by 3-Mercaptopicolinic Acid.
Balan MD, Mcleod MJ, Lotosky WR, Ghaly M, Holyoak T
Biochemistry 54 5878-87 (2015)
PMID: 26322521

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 69.64 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand GTP 1 x GTP
Ligand MN 3 x MN
Ligand NA 1 x NA
Ligand 1WD 1 x 1WD
Ligand MOH 1 x MOH
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 44.407Å b: 118.742Å c: 60.248Å
α: 90° β: 110.02° γ: 90°
R-values:
R R work R free
0.17 0.168 0.197
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Dysregulation of metabolic enzymes in tumor and stromal cells: Role in oncogenesis and therapeutic opportunities.
Khan et al. (2020)
1 more

2 mentions without citation

Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution.
Nguyen et al. (2016)
1 more