› 4ywb

X-ray diffraction
1.5Å resolution

Structure of rat cytosolic pepck in complex with 3-mercaptopicolinic acid and oxalic acid

Released:
DOI: 10.2210/pdb4ywb/pdb
PDB entry 4ywb coloured by chain, front view.
PDB entry 4ywb coloured by chain, side view.
PDB entry 4ywb coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Inhibition and Allosteric Regulation of Monomeric Phosphoenolpyruvate Carboxykinase by 3-Mercaptopicolinic Acid.
Balan MD, Mcleod MJ, Lotosky WR, Ghaly M, Holyoak T
Biochemistry 54 5878-87 (2015)
PMID: 26322521

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chains: A, C
Molecule details ›
Chains: A, C
Length: 622 amino acids
Theoretical weight: 69.5 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN 4 x MN
Ligand NA 2 x NA
Ligand OXD 2 x OXD
Ligand 1WD 2 x 1WD
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 64.124Å b: 118.732Å c: 86.393Å
α: 90° β: 107.27° γ: 90°
R-values:
R R work R free
0.175 0.173 0.21
Expression system: Escherichia coli BL21

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Citations

2 review citations

Dysregulation of metabolic enzymes in tumor and stromal cells: Role in oncogenesis and therapeutic opportunities.
Khan et al. (2020)
1 more