› 4yx9

X-ray diffraction
1.75Å resolution

Crystal structure of the CFTR inhibitory factor Cif bound to tiratricol

Released:
DOI: 10.2210/pdb4yx9/pdb
PDB entry 4yx9 coloured by chain, front view.
PDB entry 4yx9 coloured by chain, side view.
PDB entry 4yx9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Inhibiting an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa Protects CFTR.
Bahl CD, Hvorecny KL, Bomberger JM, Stanton BA, Hammock BD, Morisseau C, Madden DR
Angew Chem Int Ed Engl 54 9881-5 (2015)
PMID: 26136396

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-494541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 34.16 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3KL26 (Residues: 1-295; Coverage: 98%)
Gene name: PA14_26090
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 4HY 4 x 4HY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 169.628Å b: 84.016Å c: 89.522Å
α: 90° β: 100.48° γ: 90°
R-values:
R R work R free
0.165 0.164 0.195
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Effects of Pseudomonas aeruginosa on CFTR chloride secretion and the host immune response.
Stanton BA. (2017)
2 more