› 4z7m

X-ray diffraction
1.43Å resolution

Novel Inhibitors of Bacterial Methionine Aminopeptidase with Broad-Spectrum Biochemical Activity

Released:
DOI: 10.2210/pdb4z7m/pdb
PDB entry 4z7m coloured by chain, front view.
PDB entry 4z7m coloured by chain, side view.
PDB entry 4z7m coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Rose JA, Lahiri SD, McKinney DC, Albert R, Morningstar M, Shapiro AB, Fisher SF, Fleming PR

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AE18 (Residues: 2-262; Coverage: 99%)
Gene names: JW0163, b0168, map
Sequence domains: Metallopeptidase family M24
Structure domains: Creatinase/methionine aminopeptidase superfamily

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand 4L9 2 x 4L9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 61.985Å b: 62.712Å c: 72.978Å
α: 90° β: 89.59° γ: 90°
R-values:
R R work R free
0.254 0.251 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links