› 4z7y

X-ray diffraction
2.7Å resolution

diphosphomevalonate decarboxylase from the Sulfolobus solfataricus, space group P21

Released:
DOI: 10.2210/pdb4z7y/pdb
PDB entry 4z7y coloured by chain, front view.
PDB entry 4z7y coloured by chain, side view.
PDB entry 4z7y coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
In Vivo Formation of the Protein Disulfide Bond That Enhances the Thermostability of Diphosphomevalonate Decarboxylase, an Intracellular Enzyme from the Hyperthermophilic Archaeon Sulfolobus solfataricus.
Hattori A, Unno H, Goda S, Motoyama K, Yoshimura T, Hemmi H
J Bacteriol 197 3463-71 (2015)
PMID: 26303832

Function and Biology Details

Reaction catalysed: 
ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphosphomevalonate decarboxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 324 amino acids
Theoretical weight: 36.95 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97UL5 (Residues: 2-325; Coverage: 100%)
Gene name: SSO2989
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 94.9Å b: 154.39Å c: 109.969Å
α: 90° β: 114.41° γ: 90°
R-values:
R R work R free
0.255 0.254 0.277
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation.
Eichler et al. (2017)

2 mentions without citation

A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase.
Motoyama et al. (2017)
1 more