› 4zav

X-ray diffraction
1.4Å resolution

UbiX in complex with a covalent adduct between dimethylallyl monophosphate and reduced FMN

Released:
DOI: 10.2210/pdb4zav/pdb
PDB entry 4zav coloured by chain, front view.
PDB entry 4zav coloured by chain, side view.
PDB entry 4zav coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis.
White MD, Payne KA, Fisher K, Marshall SA, Parker D, Rattray NJ, Trivedi DK, Goodacre R, Rigby SE, Scrutton NS, Hay S, Leys D
OpenAccess logo Nature 522 502-6 (2015)
PMID: 26083743

Function and Biology Details

Reaction catalysed: 
Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin prenyltransferase UbiX Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 22.38 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HX08 (Residues: 1-209; Coverage: 100%)
Gene names: PA4019, ubiX
Sequence domains: Flavoprotein
Structure domains: Flavin prenyltransferase-like

Ligands and Environments


Cofactor: Ligand 4LS 1 x 4LS
3 bound ligands:
Ligand SCN 4 x SCN
Ligand NA 1 x NA
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: F23
Unit cell:
a: 142.18Å b: 142.18Å c: 142.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.099 0.098 0.129
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Biochemistry of Mitochondrial Coenzyme Q Biosynthesis.
Stefely et al. (2017)
16 more

3 mentions without citation

N5 Is the New C4a: Biochemical Functionalization of Reduced Flavins at the N5 Position.
Beaupre et al. (2020)
2 more