› 4zew

X-ray diffraction
1.9Å resolution

Crystal structure of PfHAD1 in complex with glucose-6-phosphate

Released:
DOI: 10.2210/pdb4zew/pdb
PDB entry 4zew coloured by chain, front view.
PDB entry 4zew coloured by chain, side view.
PDB entry 4zew coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Cap-domain closure enables diverse substrate recognition by the C2-type haloacid dehalogenase-like sugar phosphatase Plasmodium falciparum HAD1.
Park J, Guggisberg AM, Odom AR, Tolia NH
Acta Crystallogr D Biol Crystallogr 71 1824-34 (2015)
PMID: 26327372

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloacid dehalogenase-like hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 33.87 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IJ74 (Residues: 1-288; Coverage: 100%)
Gene name: PF3D7_1033400
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand G6P 1 x G6P
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 77.7Å b: 44.5Å c: 84.8Å
α: 90° β: 101.4° γ: 90°
R-values:
R R work R free
0.181 0.179 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Suppression of Drug Resistance Reveals a Genetic Mechanism of Metabolic Plasticity in Malaria Parasites.
Guggisberg et al. (2018)
4 more

3 mentions without citation

Cap-domain closure enables diverse substrate recognition by the C2-type haloacid dehalogenase-like sugar phosphatase Plasmodium falciparum HAD1.
Park et al. (2015)
2 more