› › Function and Biology

Double Mutant H80A/H81A of Fe-Type Nitrile Hydratase from Comamonas testosteroni Ni1

Source organism: Comamonas testosteroni
Biochemical function: indole-3-acetonitrile nitrile hydratase activity
Biological process: not assigned
Cellular component: not assigned

Quick links

EC 4.2.1.84: Nitrile hydratase

Reaction catalysed:
An aliphatic amide = a nitrile + H(2)O
Systematic name:
Aliphatic-amide hydro-lyase (nitrile-forming)
Alternative Name(s):
  • 3-cyanopyridine hydratase
  • Acrylonitrile hydratase
  • Aliphatic nitrile hydratase
  • H-NHase
  • L-NHase
  • NHase
  • Nitrilase
  • Nitrile hydro-lyase

GO terms

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF02211
Domain description: Nitrile hydratase beta subunit, C-terminal
Occurring in:
  1. Nitrile hydratase subunit beta
The deposited structure of PDB entry 4zgj contains 8 copies of Pfam domain PF02211 (Nitrile hydratase beta subunit) in Nitrile hydratase subunit beta. Showing 1 copy in chain B.

PF21006
Domain description: Nitrile hydratase beta subunit, N-terminal
Occurring in:
  1. Nitrile hydratase subunit beta

PF02979
Domain description: Nitrile hydratase, alpha chain
Occurring in:
  1. Nitrile hydratase alpha/Thiocyanate hydrolase gamma domain-containing protein
The deposited structure of PDB entry 4zgj contains 8 copies of Pfam domain PF02979 (Nitrile hydratase, alpha chain) in Nitrile hydratase alpha /Thiocyanate hydrolase gamma domain-containing protein. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR003168
Domain description: Nitrile hydratase, beta subunit
Occurring in:
  1. Nitrile hydratase subunit beta
IPR036648
Domain description: Nitrile hydratase alpha /Thiocyanate hydrolase gamma superfamily
Occurring in:
  1. Nitrile hydratase alpha/Thiocyanate hydrolase gamma domain-containing protein
IPR042262
Domain description: Nitrile hydratase beta subunit, N-terminal
Occurring in:
  1. Nitrile hydratase subunit beta
IPR024690
Domain description: Nitrile hydratase beta subunit domain
Occurring in:
  1. Nitrile hydratase subunit beta
IPR008990
Domain description: Electron transport accessory-like domain superfamily
Occurring in:
  1. Nitrile hydratase subunit beta
IPR004232
Domain description: Nitrile hydratase alpha/Thiocyanate hydrolase gamma
Occurring in:
  1. Nitrile hydratase alpha/Thiocyanate hydrolase gamma domain-containing protein
IPR023900
Domain description: Nitrile hydratase alpha subunit /Thiocyanate hydrolase gamma subunit
Occurring in:
  1. Nitrile hydratase alpha/Thiocyanate hydrolase gamma domain-containing protein

Structure domains

CATH CATH domains
2.30.30.50
Class: Mainly Beta
Architecture: Roll
Topology: SH3 type barrels.
Homology: SH3 type barrels.
Occurring in:
  1. Nitrile hydratase subunit beta
The deposited structure of PDB entry 4zgj contains 8 copies of CATH domain 2.30.30.50 (SH3 type barrels.) in Nitrile hydratase subunit beta. Showing 1 copy in chain B.
1.10.472.20
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Cyclin A; domain 1
Homology: Nitrile hydratase, beta subunit
Occurring in:
  1. Nitrile hydratase subunit beta
The deposited structure of PDB entry 4zgj contains 8 copies of CATH domain 1.10.472.20 (Cyclin A; domain 1) in Nitrile hydratase subunit beta. Showing 1 copy in chain B.
3.90.330.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Nitrile Hydratase; Chain A
Homology: Nitrile hydratase alpha /Thiocyanate hydrolase gamma
Occurring in:
  1. Nitrile hydratase alpha/Thiocyanate hydrolase gamma domain-containing protein
The deposited structure of PDB entry 4zgj contains 8 copies of CATH domain 3.90.330.10 (Nitrile Hydratase; Chain A) in Nitrile hydratase alpha /Thiocyanate hydrolase gamma domain-containing protein. Showing 1 copy in chain A.

Quick links