› 4zn5

X-ray diffraction
1.12Å resolution

YopH W354Y Yersinia enterocolitica PTPase bond with Divanadate glycerol ester in the active site

Released:
DOI: 10.2210/pdb4zn5/pdb
PDB entry 4zn5 coloured by chain, front view.
PDB entry 4zn5 coloured by chain, side view.
PDB entry 4zn5 coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
Conservative tryptophan mutants of the protein tyrosine phosphatase YopH exhibit impaired WPD-loop function and crystallize with divanadate esters in their active sites.
Moise G, Gallup NM, Alexandrova AN, Hengge AC, Johnson SJ
Biochemistry 54 6490-500 (2015)
PMID: 26445170

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase YopH Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.53 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli
UniProt:
  • Canonical: P15273 (Residues: 164-468; Coverage: 65%)
Gene names: yop51, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

4 bound ligands:
Ligand DVG 1 x DVG
Ligand VO4 1 x VO4
Ligand ACT 1 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 54.359Å b: 58.003Å c: 90.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.129 0.145
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The metal face of protein tyrosine phosphatase 1B.
Bellomo et al. (2016)
4 other articles

2 mentions without citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
1 more