4zq0

X-ray diffraction
3.1Å resolution

crystal structure of Giardia 14-3-3 in complex with the phosphopeptide A8Ap

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-122860 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 234 amino acids
Theoretical weight: 27.19 KDa
Source organism: Giardia lamblia P15
Expression system: Escherichia coli
UniProt:
  • Canonical: E2RU97 (Residues: 5-238; Coverage: 94%)
Gene names: GL50803_006430, GL50803_6430
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
A8Ap phosphopeptide Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 8 amino acids
Theoretical weight: 795 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 69.958Å b: 109.408Å c: 78.568Å
α: 90° β: 92.64° γ: 90°
R-values:
R R work R free
0.207 0.203 0.266
Expression systems:
  • Escherichia coli
  • Not provided