› 4zsf

X-ray diffraction
1.8Å resolution

Crystal structure of pre-specific restriction endonuclease BsaWI-DNA complex

Released:
DOI: 10.2210/pdb4zsf/pdb
PDB entry 4zsf coloured by chain, front view.
PDB entry 4zsf coloured by chain, side view.
PDB entry 4zsf coloured by chain, top view.
Source organisms:
Primary publication:
Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI.
Tamulaitis G, Rutkauskas M, Zaremba M, Grazulis S, Tamulaitiene G, Siksnys V
OpenAccess logo Nucleic Acids Res 43 8100-10 (2015)
PMID: 26240380

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-509184 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
BsaWI restriction endonuclease type 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 31.96 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: Q6UQ65 (Residues: 1-272; Coverage: 100%)
Gene name: bsaWIR
Sequence domains: BsaWI restriction endonuclease type 2
DNA Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SIN 2 x SIN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: C2221
Unit cell:
a: 89.562Å b: 136.336Å c: 73.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.152 0.188
Expression systems:
  • Escherichia coli str. K-12 substr. DH10B
  • Not provided

Quick links

Citations

4 citation in other articles

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA.
Tamulaitiene et al. (2017)
3 more

2 mentions without citation

Identification of SUMO modification sites in the base excision repair protein, Ntg1.
Swartzlander et al. (2016)
1 more