› 5a31

Electron Microscopy
4.3Å resolution

Structure of the human APC-Cdh1-Hsl1-UbcH10 complex.

Released:
DOI: 10.2210/pdb5a31/pdb
PDB entry 5a31 coloured by chain, front view.
PDB entry 5a31 coloured by chain, side view.
PDB entry 5a31 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Atomic structure of the APC/C and its mechanism of protein ubiquitination.
Chang L, Zhang Z, Yang J, McLaughlin SH, Barford D
Nature 522 450-454 (2015)
PMID: 26083744
Related structures: EMD-2925

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-126386 (preferred)
Entry contents:
20 distinct polypeptide molecules
Macromolecules (20 distinct):
Anaphase-promoting complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1441 amino acids
Theoretical weight: 159.62 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H1A4 (Residues: 11-1897; Coverage: 73%)
Gene names: ANAPC1, TSG24
Sequence domains:
Anaphase-promoting complex subunit 11 Chain: B
Molecule details ›
Chain: B
Length: 84 amino acids
Theoretical weight: 9.87 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NYG5 (Residues: 1-84; Coverage: 100%)
Gene names: ANAPC11, HSPC214
Sequence domains: Anaphase-promoting complex subunit 11 RING-H2 finger
Cell division cycle protein 23 homolog Chains: C, P
Anaphase-promoting complex subunit 15 Chain: D
Anaphase-promoting complex subunit 16 Chain: E
Cell division cycle protein 27 homolog Chains: F, H
Anaphase-promoting complex subunit CDC26 Chains: G, W
Anaphase-promoting complex subunit 4 Chain: I
Cell division cycle protein 16 homolog Chain: J
Cell division cycle protein 16 homolog Chain: K
Anaphase-promoting complex subunit 10 Chain: L
Anaphase-promoting complex subunit 13 Chain: M
Anaphase-promoting complex subunit 2 Chain: N
Anaphase-promoting complex subunit 5 Chain: O
Ubiquitin-conjugating enzyme E2 C Chain: Q
APC/C activator protein CDH1 Chain: R
THE ANAPHASE-PROMOTING COMPLEX CHAIN T Chain: T
THE ANAPHASE-PROMOTING COMPLEX CHAIN U Chain: U
THE ANAPHASE-PROMOTING COMPLEX CHAIN V Chain: V
Anaphase-promoting complex subunit 7 Chains: X, Y

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-2925
Expression system: Trichoplusia ni

Quick links

Citations

27 review citations

The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics.
Musacchio A. (2015)
26 more

7 mentions without citation

Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C).
Alfieri et al. (2017)
6 more