› 5aeg

X-ray diffraction
1.85Å resolution

A bacterial protein structure in glycoside hydrolase family 31.

Released:
DOI: 10.2210/pdb5aeg/pdb
PDB entry 5aeg coloured by chain, front view.
PDB entry 5aeg coloured by chain, side view.
PDB entry 5aeg coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
YihQ is a sulfoquinovosidase that cleaves sulfoquinovosyl diacylglyceride sulfolipids.
Speciale G, Jin Y, Davies GJ, Williams SJ, Goddard-Borger ED
Nat Chem Biol 12 215-7 (2016)
PMID: 26878550

Function and Biology Details

Reaction catalysed: 
A 6-sulfo-alpha-D-quinovosyl diacylglycerol + H(2)O = 6-sulfo-alpha-D-quinovose + a 1,2-diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfoquinovosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 686 amino acids
Theoretical weight: 78.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32138 (Residues: 1-678; Coverage: 100%)
Gene names: JW3849, b3878, squQ, yihQ
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand CA 3 x CA
Ligand CL 2 x CL
Ligand MPD 3 x MPD
Ligand B9D 1 x B9D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 78.665Å b: 112.643Å c: 111.881Å
α: 90° β: 109.52° γ: 90°
R-values:
R R work R free
0.162 0.16 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Sulfur Cycling and the Intestinal Microbiome.
Barton et al. (2017)
2 more