5an9

Electron Microscopy
3.3Å resolution

Mechanism of eIF6 release from the nascent 60S ribosomal subunit

Released:
Related structures: EMD-3145

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-147098 (preferred)
Entry contents:
10 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (11 distinct):
Large ribosomal subunit protein uL3 Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 45.16 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P34113 (Residues: 1-398; Coverage: 100%)
Gene names: DDB_G0291862, rpl3
Sequence domains: Ribosomal protein L3
Structure domains:
Large ribosomal subunit protein uL6 Chain: B
Molecule details ›
Chain: B
Length: 188 amino acids
Theoretical weight: 21.25 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: Q54XI5 (Residues: 1-188; Coverage: 100%)
Gene names: DDB_G0278959, rpl9
Sequence domains: Ribosomal protein L6
Structure domains: Ribosomal protein L6, alpha-beta domain
Large ribosomal subunit protein uL10 Chain: C
Large ribosomal subunit protein uL11 Chain: D
Large ribosomal subunit protein uL14 Chain: E
Large ribosomal subunit protein uL16 Chain: F
Large ribosomal subunit protein eL24 Chain: G
Large ribosomal subunit protein eL40 Chain: H
Eukaryotic translation initiation factor 6 Chain: I
Ribosome maturation protein SBDS Chain: J
26S RIBOSOMAL RNA Chain: N

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-3145
Expression system: Escherichia coli BL21(DE3)