› 5an9

Electron Microscopy
3.3Å resolution

Mechanism of eIF6 release from the nascent 60S ribosomal subunit

Released:
DOI: 10.2210/pdb5an9/pdb
PDB entry 5an9 coloured by chain, front view.
PDB entry 5an9 coloured by chain, side view.
PDB entry 5an9 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of eIF6 release from the nascent 60S ribosomal subunit.
Weis F, Giudice E, Churcher M, Jin L, Hilcenko C, Wong CC, Traynor D, Kay RR, Warren AJ
Nat Struct Mol Biol 22 914-9 (2015)
PMID: 26479198
Related structures: EMD-3145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-147098 (preferred)
Entry contents:
10 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (11 distinct):
Large ribosomal subunit protein uL3 Chain: A
Molecule details ›
Chain: A
Length: 398 amino acids
Theoretical weight: 45.16 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P34113 (Residues: 1-398; Coverage: 100%)
Gene names: DDB_G0291862, rpl3
Sequence domains: Ribosomal protein L3
Structure domains:
Large ribosomal subunit protein uL6 Chain: B
Molecule details ›
Chain: B
Length: 188 amino acids
Theoretical weight: 21.25 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: Q54XI5 (Residues: 1-188; Coverage: 100%)
Gene names: DDB_G0278959, rpl9
Sequence domains: Ribosomal protein L6
Structure domains: Ribosomal protein L6, alpha-beta domain
Large ribosomal subunit protein uL10 Chain: C
Large ribosomal subunit protein uL11 Chain: D
Large ribosomal subunit protein uL14 Chain: E
Large ribosomal subunit protein uL16 Chain: F
Large ribosomal subunit protein eL24 Chain: G
Large ribosomal subunit protein eL40 Chain: H
Eukaryotic translation initiation factor 6 Chain: I
Ribosome maturation protein SBDS Chain: J
26S RIBOSOMAL RNA Chain: N

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.3Å
Relevant EMDB volumes: EMD-3145
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Ribosome assembly coming into focus.
Klinge et al. (2019)
39 more

6 mentions without citation

Mechanistic insight into eukaryotic 60S ribosomal subunit biogenesis by cryo-electron microscopy.
Greber BJ. (2016)
5 more