› 5b2c

X-ray diffraction
2.24Å resolution

Crystal structure of Mumps virus hemagglutinin-neuraminidase

Released:
DOI: 10.2210/pdb5b2c/pdb
PDB entry 5b2c coloured by chain, front view.
PDB entry 5b2c coloured by chain, side view.
PDB entry 5b2c coloured by chain, top view.
Source organism: Mumps orthorubulavirus
Primary publication:
Trisaccharide containing α2,3-linked sialic acid is a receptor for mumps virus.
Kubota M, Takeuchi K, Watanabe S, Ohno S, Matsuoka R, Kohda D, Nakakita SI, Hiramatsu H, Suzuki Y, Nakayama T, Terada T, Shimizu K, Shimizu N, Shiroishi M, Yanagi Y, Hashiguchi T
Proc Natl Acad Sci U S A 113 11579-11584 (2016)
PMID: 27671656

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HN protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 489 amino acids
Theoretical weight: 54.27 KDa
Source organism: Mumps orthorubulavirus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9WAF5 (Residues: 106-582; Coverage: 82%)
Gene name: HN
Sequence domains: Haemagglutinin-neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

2 bound ligands:
Ligand NAG 8 x NAG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P61
Unit cell:
a: 137.523Å b: 137.523Å c: 178.269Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.222
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Sialic Acid Receptors: The Key to Solving the Enigma of Zoonotic Virus Spillover.
Kuchipudi et al. (2021)
7 more

12 mentions without citation

Receptor-mediated cell entry of paramyxoviruses: Mechanisms, and consequences for tropism and pathogenesis.
Navaratnarajah et al. (2020)
11 more