› 5cht

X-ray diffraction
2.8Å resolution

Crystal structure of USP18

Released:
DOI: 10.2210/pdb5cht/pdb
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PDB entry 5cht coloured by chain, front view.
PDB entry 5cht coloured by chain, side view.
PDB entry 5cht coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of the specificity of USP18 toward ISG15.
Basters A, Geurink PP, Röcker A, Witting KF, Tadayon R, Hess S, Semrau MS, Storici P, Ovaa H, Knobeloch KP, Fritz G
OpenAccess logo Nat Struct Mol Biol 24 270-278 (2017)
PMID: 28165509

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubl carboxyl-terminal hydrolase 18 Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 37.56 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9WTV6 (Residues: 46-368; Coverage: 88%)
Gene names: Ubp43, Usp18
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 53.959Å b: 89.747Å c: 149.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.283
Expression system: Spodoptera frugiperda

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Citations

15 review citations

Mechanisms of Deubiquitinase Specificity and Regulation.
Mevissen et al. (2017)
14 more

6 mentions without citation

USP18 - a multifunctional component in the interferon response.
Basters et al. (2018)
5 more

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