› 5dcm

X-ray diffraction
1.6Å resolution

Structure of a lantibiotic response regulator: C-terminal domain of the nisin resistance regulator NsrR

Released:
DOI: 10.2210/pdb5dcm/pdb
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Source organism: Streptococcus agalactiae
Primary publication:
Structure of the Response Regulator NsrR from Streptococcus agalactiae, Which Is Involved in Lantibiotic Resistance.
Khosa S, Hoeppner A, Gohlke H, Schmitt L, Smits SH
OpenAccess logo PLoS One 11 e0149903 (2016)
PMID: 26930060

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197875 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Response regulator transcription factor Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.76 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: X5JZS1 (Residues: 1-222; Coverage: 100%)
Gene names: AX245_04450, C4618_08650, NCTC8184_01626, QP229_02540, graR
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Response regulator transcription factor Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 27.76 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: X5JZS1 (Residues: 1-222; Coverage: 100%)
Gene names: AX245_04450, C4618_08650, NCTC8184_01626, QP229_02540, graR
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 56.38Å b: 60.46Å c: 56.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.219
Expression system: Escherichia coli

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Citations

5 review citations

Group B Streptococcal Maternal Colonization and Neonatal Disease: Molecular Mechanisms and Preventative Approaches.
Patras et al. (2018)
4 more

1 mention without citation

Structure of the Response Regulator NsrR from Streptococcus agalactiae, Which Is Involved in Lantibiotic Resistance.
Khosa et al. (2016)

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