› 5dfw

X-ray diffraction
2.33Å resolution

CRYSTAL STRUCTURE OF HUMAN CD81 LARGE EXTRACELLULAR LOOP IN COMPLEX WITH SINGLE CHAIN FV FRAGMENT K13

Released:
DOI: 10.2210/pdb5dfw/pdb
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PDB entry 5dfw coloured by chain, front view.
PDB entry 5dfw coloured by chain, side view.
PDB entry 5dfw coloured by chain, top view.
Source organisms:
Primary publication:
VH-VL orientation prediction for antibody humanization candidate selection: A case study.
Bujotzek A, Lipsmeier F, Harris SF, Benz J, Kuglstatter A, Georges G
MAbs 8 288-305 (2016)
PMID: 26637054

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211406 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CD81 antigen Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 10.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60033 (Residues: 112-201; Coverage: 38%)
Gene names: CD81, TAPA1, TSPAN28
Sequence domains: Tetraspanin family
Structure domains: Tetraspanin
SINGLE CHAIN FV FRAGMENT Chain: H
Molecule details ›
Chain: H
Length: 243 amino acids
Theoretical weight: 25.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P2
Unit cell:
a: 49.652Å b: 49.671Å c: 80.452Å
α: 90° β: 105.3° γ: 90°
R-values:
R R work R free
0.194 0.19 0.264
Expression system: Escherichia coli

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Citations

5 review citations

Understanding the Significance and Implications of Antibody Numbering and Antigen-Binding Surface/Residue Definition.
Dondelinger et al. (2018)
4 more

3 mentions without citation

A dynamic interaction between CD19 and the tetraspanin CD81 controls B cell co-receptor trafficking.
Susa et al. (2020)
2 more

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