› 5dlq

X-ray diffraction
3.2Å resolution

Crystal structure of RanGTP-Exportin 4-eIF5A complex

Released:
DOI: 10.2210/pdb5dlq/pdb
PDB entry 5dlq coloured by chain, front view.
PDB entry 5dlq coloured by chain, side view.
PDB entry 5dlq coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A.
Aksu M, Trakhanov S, Görlich D
OpenAccess logo Nat Commun 7 11952 (2016)
PMID: 27306458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158720 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Exportin-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1113 amino acids
Theoretical weight: 125.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q9ESJ0 (Residues: 1-1151; Coverage: 97%)
Gene names: Kiaa1721, Xpo4
Sequence domains: CRM1 C terminal
GTP-binding nuclear protein Ran Chains: C, D
Molecule details ›
Chains: C, D
Length: 176 amino acids
Theoretical weight: 20.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P62826 (Residues: 5-180; Coverage: 82%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Eukaryotic translation initiation factor 5A-1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 141 amino acids
Theoretical weight: 15.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63241 (Residues: 15-154; Coverage: 91%)
Gene name: EIF5A
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
1 modified residue:
Ligand 5CT 2 x 5CT

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 98.616Å b: 98.616Å c: 726.864Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.27
Expression systems:
  • Escherichia coli K-12
  • Escherichia coli BL21

Quick links

Citations

14 review citations

Hypusine, a polyamine-derived amino acid critical for eukaryotic translation.
Park et al. (2018)
13 more

6 mentions without citation

Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing.
Arpat et al. (2020)
5 more