› 5dze

X-ray diffraction
0.97Å resolution

Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with cellotetraose

Released:
DOI: 10.2210/pdb5dze/pdb
PDB entry 5dze coloured by chain, front view.
PDB entry 5dze coloured by chain, side view.
PDB entry 5dze coloured by chain, top view.
Source organism: Vitis vinifera
Primary publication:
Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases.
McGregor N, Yin V, Tung CC, Van Petegem F, Brumer H
Plant J 89 651-670 (2017)
PMID: 27859885

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-514569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.24 KDa
Source organism: Vitis vinifera
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F6I323 (Residues: 1-208; Coverage: 100%)
Gene name: VIT_15s0048g01850
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
1 bound ligand:
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 45.15Å b: 52.02Å c: 83.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.122 0.137
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Reconsidering the function of the xyloglucan endotransglucosylase/hydrolase family.
Ishida et al. (2022)
2 more

4 mentions without citation

Crystallographic insight into the evolutionary origins of xyloglucan endotransglycosylases and endohydrolases.
McGregor et al. (2017)
3 more