5epe

X-ray diffraction
1.9Å resolution

Crystal structure of SAM-dependent methyltransferase from Thiobacillus denitrificans in complex with S-Adenosyl-L-homocysteine

Released:
Model geometry
Fit model/data
Entry authors: LaRowe C, Shabalin IG, Kutner J, Handing KB, Stead M, Hillerich BS, Ahmed M, Seidel R, Bonanno J, Almo SC, Minor W, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-174784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 27.45 KDa
Source organism: Thiobacillus denitrificans ATCC 25259
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3SJX0 (Residues: 1-248; Coverage: 100%)
Gene name: Tbd_1076
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: F23
Unit cell:
a: 157.536Å b: 157.536Å c: 157.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.137 0.136 0.167
Expression system: Escherichia coli BL21(DE3)

PDB-REDO

Experimental raw data