› 5fn8

X-ray diffraction
2.45Å resolution

Crystal structure of rat CD45 extracellular region, domains d3-d4

Released:
DOI: 10.2210/pdb5fn8/pdb
PDB entry 5fn8 coloured by chain, front view.
PDB entry 5fn8 coloured by chain, side view.
PDB entry 5fn8 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Initiation of T cell signaling by CD45 segregation at 'close contacts'.
Chang VT, Fernandes RA, Ganzinger KA, Lee SF, Siebold C, McColl J, Jönsson P, Palayret M, Harlos K, Coles CH, Jones EY, Lui Y, Huang E, Gilbert RJC, Klenerman D, Aricescu AR, Davis SJ
OpenAccess logo Nat Immunol 17 574-582 (2016)
PMID: 26998761

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Receptor-type tyrosine-protein phosphatase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.44 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04157 (Residues: 357-546; Coverage: 15%)
Gene name: Ptprc
Sequence domains: Fibronectin type III domain

Ligands and Environments

2 bound ligands:
Ligand NAG 6 x NAG
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I222
Unit cell:
a: 84.41Å b: 131.16Å c: 141.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.236
Expression system: Cricetulus griseus

Quick links

Citations

52 review citations

TCR Signaling: Mechanisms of Initiation and Propagation.
Courtney et al. (2018)
51 more

1 mention without citation

Initiation of T cell signaling by CD45 segregation at 'close contacts'.
Chang et al. (2016)