› 5fno

X-ray diffraction
2.04Å resolution

Manganese Lipoxygenase

Released:
DOI: 10.2210/pdb5fno/pdb
PDB entry 5fno coloured by chain, front view.
PDB entry 5fno coloured by chain, side view.
PDB entry 5fno coloured by chain, top view.
Source organism: Pyricularia oryzae
Primary publication:
Crystal Structure of Manganese Lipoxygenase of the Rice Blast Fungus Magnaporthe oryzae.
Wennman A, Oliw EH, Karkehabadi S, Chen Y
J Biol Chem 291 8130-9 (2016)
PMID: 26783260

Function and Biology Details

Reactions catalysed: 
Linoleate + O(2) = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Manganese lipoxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 605 amino acids
Theoretical weight: 67.06 KDa
Source organism: Pyricularia oryzae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: G4NAP4 (Residues: 17-588; Coverage: 95%)
Gene name: MGG_08499
Sequence domains: Lipoxygenase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand MN 2 x MN
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 70.725Å b: 111.374Å c: 171.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.213
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Oxidative Stress in the Male Germline: A Review of Novel Strategies to Reduce 4-Hydroxynonenal Production.
Walters et al. (2018)
19 other articles

4 mentions without citation

Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases.
Offenbacher et al. (2020)
3 more