› 5g5s

X-ray diffraction
2.29Å resolution

Structure of the Argonaute protein from Methanocaldcoccus janaschii

Released:
DOI: 10.2210/pdb5g5s/pdb
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PDB entry 5g5s coloured by chain, front view.
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PDB entry 5g5s coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural and mechanistic insights into an archaeal DNA-guided Argonaute protein.
Willkomm S, Oellig CA, Zander A, Restle T, Keegan R, Grohmann D, Schneider S
Nat Microbiol 2 17035 (2017)
PMID: 28319084

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute Chain: A
Molecule details ›
Chain: A
Length: 715 amino acids
Theoretical weight: 84.96 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q58717 (Residues: 1-713; Coverage: 100%)
Gene names: MJ1321, ago
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 63.477Å b: 104.932Å c: 115.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.236
Expression system: Escherichia coli BL21

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Citations

11 review citations

Prokaryotic Argonaute proteins: novel genome-editing tools?
Hegge et al. (2018)
10 more

2 mentions without citation

DNA silencing by prokaryotic Argonaute proteins adds a new layer of defense against invading nucleic acids.
Willkomm et al. (2018)
1 more

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